Review



nucleofections k562  (ATCC)


Bioz Verified Symbol ATCC is a verified supplier
Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 99
      Buy from Supplier

    Structured Review

    ATCC nucleofections k562
    Synthesized and chemically modified sgRNAs facilitate high frequencies of indels and HR in the human cell line <t>K562.</t> (a) Sequence and schematic secondary structure of the IL2RG sgRNA loaded into Cas9 and bound to its genomic target site. Nucleotides with chemical modifications are marked with red flags. (b) Structures of chemical modifications incorporated during chemical synthesis of sgRNAs (sequences can be found in Supplementary Table 1). (c,d amplicons (c) or gene addition by HR at the three loci IL2RG, HBB and CCR5 with synthetic sgRNAs (d). The synthetic sgRNAs were delivered at 1 μg (light shade) or 20 μg (dark shade) per 1 million cells. Cas9 was expressed from a plasmid (2 μg) and for HR experiments 5 μg of GFP-encoding donor plasmid was included. As a positive control, 2 μg of sgRNA plasmid encoding both the sgRNA and the Cas9 protein was used (gray bars). Bars represent average values + s.e.m., n = 3. (e) Specificity of targeted cleavage mediated by synthetic sgRNAs as performed in c for 20 μg of sgRNA. Indel frequencies were measured by deep sequencing of PCR amplicons of the targeted genomic loci and three bioinformatically predicted off-target loci for each gene. Bars represent average values + s.e.m., n = 3. (f) Staggered delivery of 15 μg Cas9 mRNA and 10 μg IL2RG synthetic sgRNAs into 1 million K562 cells using electroporation. Bars represent average indel frequencies + s.e.m., n = 3, as measured by tracking of indels by decomposition (TIDE) analysis of PCR amplicons spanning the sgRNA target sites, using a mock-treated sample as reference control. (g) Cas9 protein was complexed with a 2.5 molar excess of the indicated synthetic IL2RG sgRNAs and nucleofected into 1 million K562 cells at the indicated amounts. Indel frequencies were measured by TIDE analysis as above and bars represent average indel frequencies + s.e.m., n = 3.
    Nucleofections K562, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 10773 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nucleofections+k562/pmc04729442-157-3-5?v=ATCC
    Average 99 stars, based on 10773 article reviews
    nucleofections k562 - by Bioz Stars, 2026-07
    99/100 stars

    Images

    1) Product Images from "Chemically modified guide RNAs enhance CRISPR-Cas genome editing in human primary cells"

    Article Title: Chemically modified guide RNAs enhance CRISPR-Cas genome editing in human primary cells

    Journal: Nature biotechnology

    doi: 10.1038/nbt.3290

    Synthesized and chemically modified sgRNAs facilitate high frequencies of indels and HR in the human cell line K562. (a) Sequence and schematic secondary structure of the IL2RG sgRNA loaded into Cas9 and bound to its genomic target site. Nucleotides with chemical modifications are marked with red flags. (b) Structures of chemical modifications incorporated during chemical synthesis of sgRNAs (sequences can be found in Supplementary Table 1). (c,d amplicons (c) or gene addition by HR at the three loci IL2RG, HBB and CCR5 with synthetic sgRNAs (d). The synthetic sgRNAs were delivered at 1 μg (light shade) or 20 μg (dark shade) per 1 million cells. Cas9 was expressed from a plasmid (2 μg) and for HR experiments 5 μg of GFP-encoding donor plasmid was included. As a positive control, 2 μg of sgRNA plasmid encoding both the sgRNA and the Cas9 protein was used (gray bars). Bars represent average values + s.e.m., n = 3. (e) Specificity of targeted cleavage mediated by synthetic sgRNAs as performed in c for 20 μg of sgRNA. Indel frequencies were measured by deep sequencing of PCR amplicons of the targeted genomic loci and three bioinformatically predicted off-target loci for each gene. Bars represent average values + s.e.m., n = 3. (f) Staggered delivery of 15 μg Cas9 mRNA and 10 μg IL2RG synthetic sgRNAs into 1 million K562 cells using electroporation. Bars represent average indel frequencies + s.e.m., n = 3, as measured by tracking of indels by decomposition (TIDE) analysis of PCR amplicons spanning the sgRNA target sites, using a mock-treated sample as reference control. (g) Cas9 protein was complexed with a 2.5 molar excess of the indicated synthetic IL2RG sgRNAs and nucleofected into 1 million K562 cells at the indicated amounts. Indel frequencies were measured by TIDE analysis as above and bars represent average indel frequencies + s.e.m., n = 3.
    Figure Legend Snippet: Synthesized and chemically modified sgRNAs facilitate high frequencies of indels and HR in the human cell line K562. (a) Sequence and schematic secondary structure of the IL2RG sgRNA loaded into Cas9 and bound to its genomic target site. Nucleotides with chemical modifications are marked with red flags. (b) Structures of chemical modifications incorporated during chemical synthesis of sgRNAs (sequences can be found in Supplementary Table 1). (c,d amplicons (c) or gene addition by HR at the three loci IL2RG, HBB and CCR5 with synthetic sgRNAs (d). The synthetic sgRNAs were delivered at 1 μg (light shade) or 20 μg (dark shade) per 1 million cells. Cas9 was expressed from a plasmid (2 μg) and for HR experiments 5 μg of GFP-encoding donor plasmid was included. As a positive control, 2 μg of sgRNA plasmid encoding both the sgRNA and the Cas9 protein was used (gray bars). Bars represent average values + s.e.m., n = 3. (e) Specificity of targeted cleavage mediated by synthetic sgRNAs as performed in c for 20 μg of sgRNA. Indel frequencies were measured by deep sequencing of PCR amplicons of the targeted genomic loci and three bioinformatically predicted off-target loci for each gene. Bars represent average values + s.e.m., n = 3. (f) Staggered delivery of 15 μg Cas9 mRNA and 10 μg IL2RG synthetic sgRNAs into 1 million K562 cells using electroporation. Bars represent average indel frequencies + s.e.m., n = 3, as measured by tracking of indels by decomposition (TIDE) analysis of PCR amplicons spanning the sgRNA target sites, using a mock-treated sample as reference control. (g) Cas9 protein was complexed with a 2.5 molar excess of the indicated synthetic IL2RG sgRNAs and nucleofected into 1 million K562 cells at the indicated amounts. Indel frequencies were measured by TIDE analysis as above and bars represent average indel frequencies + s.e.m., n = 3.

    Techniques Used: Synthesized, Modification, Sequencing, Plasmid Preparation, Positive Control, Electroporation, Control



    Similar Products

    nucleofections k562  (ATCC)
    99
    ATCC nucleofections k562
    Synthesized and chemically modified sgRNAs facilitate high frequencies of indels and HR in the human cell line <t>K562.</t> (a) Sequence and schematic secondary structure of the IL2RG sgRNA loaded into Cas9 and bound to its genomic target site. Nucleotides with chemical modifications are marked with red flags. (b) Structures of chemical modifications incorporated during chemical synthesis of sgRNAs (sequences can be found in Supplementary Table 1). (c,d amplicons (c) or gene addition by HR at the three loci IL2RG, HBB and CCR5 with synthetic sgRNAs (d). The synthetic sgRNAs were delivered at 1 μg (light shade) or 20 μg (dark shade) per 1 million cells. Cas9 was expressed from a plasmid (2 μg) and for HR experiments 5 μg of GFP-encoding donor plasmid was included. As a positive control, 2 μg of sgRNA plasmid encoding both the sgRNA and the Cas9 protein was used (gray bars). Bars represent average values + s.e.m., n = 3. (e) Specificity of targeted cleavage mediated by synthetic sgRNAs as performed in c for 20 μg of sgRNA. Indel frequencies were measured by deep sequencing of PCR amplicons of the targeted genomic loci and three bioinformatically predicted off-target loci for each gene. Bars represent average values + s.e.m., n = 3. (f) Staggered delivery of 15 μg Cas9 mRNA and 10 μg IL2RG synthetic sgRNAs into 1 million K562 cells using electroporation. Bars represent average indel frequencies + s.e.m., n = 3, as measured by tracking of indels by decomposition (TIDE) analysis of PCR amplicons spanning the sgRNA target sites, using a mock-treated sample as reference control. (g) Cas9 protein was complexed with a 2.5 molar excess of the indicated synthetic IL2RG sgRNAs and nucleofected into 1 million K562 cells at the indicated amounts. Indel frequencies were measured by TIDE analysis as above and bars represent average indel frequencies + s.e.m., n = 3.
    Nucleofections K562, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nucleofections+k562/pmc04729442-157-3-5?v=ATCC
    Average 99 stars, based on 1 article reviews
    nucleofections k562 - by Bioz Stars, 2026-07
    99/100 stars
    		  Buy from Supplier
    nucleofected k562 cells  (Amaxa)
    90
    Amaxa nucleofected k562 cells
    Synthesized and chemically modified sgRNAs facilitate high frequencies of indels and HR in the human cell line <t>K562.</t> (a) Sequence and schematic secondary structure of the IL2RG sgRNA loaded into Cas9 and bound to its genomic target site. Nucleotides with chemical modifications are marked with red flags. (b) Structures of chemical modifications incorporated during chemical synthesis of sgRNAs (sequences can be found in Supplementary Table 1). (c,d amplicons (c) or gene addition by HR at the three loci IL2RG, HBB and CCR5 with synthetic sgRNAs (d). The synthetic sgRNAs were delivered at 1 μg (light shade) or 20 μg (dark shade) per 1 million cells. Cas9 was expressed from a plasmid (2 μg) and for HR experiments 5 μg of GFP-encoding donor plasmid was included. As a positive control, 2 μg of sgRNA plasmid encoding both the sgRNA and the Cas9 protein was used (gray bars). Bars represent average values + s.e.m., n = 3. (e) Specificity of targeted cleavage mediated by synthetic sgRNAs as performed in c for 20 μg of sgRNA. Indel frequencies were measured by deep sequencing of PCR amplicons of the targeted genomic loci and three bioinformatically predicted off-target loci for each gene. Bars represent average values + s.e.m., n = 3. (f) Staggered delivery of 15 μg Cas9 mRNA and 10 μg IL2RG synthetic sgRNAs into 1 million K562 cells using electroporation. Bars represent average indel frequencies + s.e.m., n = 3, as measured by tracking of indels by decomposition (TIDE) analysis of PCR amplicons spanning the sgRNA target sites, using a mock-treated sample as reference control. (g) Cas9 protein was complexed with a 2.5 molar excess of the indicated synthetic IL2RG sgRNAs and nucleofected into 1 million K562 cells at the indicated amounts. Indel frequencies were measured by TIDE analysis as above and bars represent average indel frequencies + s.e.m., n = 3.
    Nucleofected K562 Cells, supplied by Amaxa, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nucleofections+k562/pmc04489214-280-12-7?v=Amaxa
    Average 90 stars, based on 1 article reviews
    nucleofected k562 cells - by Bioz Stars, 2026-07
    90/100 stars
    		  Buy from Supplier
    k562 nucleofection kit  (Amaxa)
    90
    Amaxa k562 nucleofection kit
    MKRN2 expression was down-regulated in CD34 + cells by <t>nucleofection</t> of shRNA. CD34 + cells expressing pGFP-V-RS-MKRN2 had lower expansion capacity in day 7 culture ( P = 0.005; n = 4) and a trend of reduced expansion at days 2 and 8, compared with cells transfected with non-effective sh-pGFP-V-RS-NE or empty vector (pGFP-V-RS).
    K562 Nucleofection Kit, supplied by Amaxa, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nucleofections+k562/pmc03968021-46-25-28?v=Amaxa
    Average 90 stars, based on 1 article reviews
    k562 nucleofection kit - by Bioz Stars, 2026-07
    90/100 stars
    		  Buy from Supplier
    nucleofected k562 cells  (Lonza)
    90
    Lonza nucleofected k562 cells
    MKRN2 expression was down-regulated in CD34 + cells by <t>nucleofection</t> of shRNA. CD34 + cells expressing pGFP-V-RS-MKRN2 had lower expansion capacity in day 7 culture ( P = 0.005; n = 4) and a trend of reduced expansion at days 2 and 8, compared with cells transfected with non-effective sh-pGFP-V-RS-NE or empty vector (pGFP-V-RS).
    Nucleofected K562 Cells, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nucleofections+k562/pmc03267864__NIHMS341298___supplement___1-337-22-23?v=Lonza
    Average 90 stars, based on 1 article reviews
    nucleofected k562 cells - by Bioz Stars, 2026-07
    90/100 stars
    		  Buy from Supplier
    sirna nucleofection of suspension cells k562  (Amaxa)
    90
    Amaxa sirna nucleofection of suspension cells k562
    MKRN2 expression was down-regulated in CD34 + cells by <t>nucleofection</t> of shRNA. CD34 + cells expressing pGFP-V-RS-MKRN2 had lower expansion capacity in day 7 culture ( P = 0.005; n = 4) and a trend of reduced expansion at days 2 and 8, compared with cells transfected with non-effective sh-pGFP-V-RS-NE or empty vector (pGFP-V-RS).
    Sirna Nucleofection Of Suspension Cells K562, supplied by Amaxa, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nucleofections+k562/pm15102540-84-25-11?v=Amaxa
    Average 90 stars, based on 1 article reviews
    sirna nucleofection of suspension cells k562 - by Bioz Stars, 2026-07
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Synthesized and chemically modified sgRNAs facilitate high frequencies of indels and HR in the human cell line K562. (a) Sequence and schematic secondary structure of the IL2RG sgRNA loaded into Cas9 and bound to its genomic target site. Nucleotides with chemical modifications are marked with red flags. (b) Structures of chemical modifications incorporated during chemical synthesis of sgRNAs (sequences can be found in Supplementary Table 1). (c,d amplicons (c) or gene addition by HR at the three loci IL2RG, HBB and CCR5 with synthetic sgRNAs (d). The synthetic sgRNAs were delivered at 1 μg (light shade) or 20 μg (dark shade) per 1 million cells. Cas9 was expressed from a plasmid (2 μg) and for HR experiments 5 μg of GFP-encoding donor plasmid was included. As a positive control, 2 μg of sgRNA plasmid encoding both the sgRNA and the Cas9 protein was used (gray bars). Bars represent average values + s.e.m., n = 3. (e) Specificity of targeted cleavage mediated by synthetic sgRNAs as performed in c for 20 μg of sgRNA. Indel frequencies were measured by deep sequencing of PCR amplicons of the targeted genomic loci and three bioinformatically predicted off-target loci for each gene. Bars represent average values + s.e.m., n = 3. (f) Staggered delivery of 15 μg Cas9 mRNA and 10 μg IL2RG synthetic sgRNAs into 1 million K562 cells using electroporation. Bars represent average indel frequencies + s.e.m., n = 3, as measured by tracking of indels by decomposition (TIDE) analysis of PCR amplicons spanning the sgRNA target sites, using a mock-treated sample as reference control. (g) Cas9 protein was complexed with a 2.5 molar excess of the indicated synthetic IL2RG sgRNAs and nucleofected into 1 million K562 cells at the indicated amounts. Indel frequencies were measured by TIDE analysis as above and bars represent average indel frequencies + s.e.m., n = 3.

    Journal: Nature biotechnology

    Article Title: Chemically modified guide RNAs enhance CRISPR-Cas genome editing in human primary cells

    doi: 10.1038/nbt.3290

    Figure Lengend Snippet: Synthesized and chemically modified sgRNAs facilitate high frequencies of indels and HR in the human cell line K562. (a) Sequence and schematic secondary structure of the IL2RG sgRNA loaded into Cas9 and bound to its genomic target site. Nucleotides with chemical modifications are marked with red flags. (b) Structures of chemical modifications incorporated during chemical synthesis of sgRNAs (sequences can be found in Supplementary Table 1). (c,d amplicons (c) or gene addition by HR at the three loci IL2RG, HBB and CCR5 with synthetic sgRNAs (d). The synthetic sgRNAs were delivered at 1 μg (light shade) or 20 μg (dark shade) per 1 million cells. Cas9 was expressed from a plasmid (2 μg) and for HR experiments 5 μg of GFP-encoding donor plasmid was included. As a positive control, 2 μg of sgRNA plasmid encoding both the sgRNA and the Cas9 protein was used (gray bars). Bars represent average values + s.e.m., n = 3. (e) Specificity of targeted cleavage mediated by synthetic sgRNAs as performed in c for 20 μg of sgRNA. Indel frequencies were measured by deep sequencing of PCR amplicons of the targeted genomic loci and three bioinformatically predicted off-target loci for each gene. Bars represent average values + s.e.m., n = 3. (f) Staggered delivery of 15 μg Cas9 mRNA and 10 μg IL2RG synthetic sgRNAs into 1 million K562 cells using electroporation. Bars represent average indel frequencies + s.e.m., n = 3, as measured by tracking of indels by decomposition (TIDE) analysis of PCR amplicons spanning the sgRNA target sites, using a mock-treated sample as reference control. (g) Cas9 protein was complexed with a 2.5 molar excess of the indicated synthetic IL2RG sgRNAs and nucleofected into 1 million K562 cells at the indicated amounts. Indel frequencies were measured by TIDE analysis as above and bars represent average indel frequencies + s.e.m., n = 3.

    Article Snippet: Cell culture and nucleofections K562 (ATCC) and T cells were cultured at 37 °C, 5% CO 2 , and ambient oxygen levels.

    Techniques: Synthesized, Modification, Sequencing, Plasmid Preparation, Positive Control, Electroporation, Control

    MKRN2 expression was down-regulated in CD34 + cells by nucleofection of shRNA. CD34 + cells expressing pGFP-V-RS-MKRN2 had lower expansion capacity in day 7 culture ( P = 0.005; n = 4) and a trend of reduced expansion at days 2 and 8, compared with cells transfected with non-effective sh-pGFP-V-RS-NE or empty vector (pGFP-V-RS).

    Journal: PLoS ONE

    Article Title: Ubiquitous Expression of MAKORIN-2 in Normal and Malignant Hematopoietic Cells and Its Growth Promoting Activity

    doi: 10.1371/journal.pone.0092706

    Figure Lengend Snippet: MKRN2 expression was down-regulated in CD34 + cells by nucleofection of shRNA. CD34 + cells expressing pGFP-V-RS-MKRN2 had lower expansion capacity in day 7 culture ( P = 0.005; n = 4) and a trend of reduced expansion at days 2 and 8, compared with cells transfected with non-effective sh-pGFP-V-RS-NE or empty vector (pGFP-V-RS).

    Article Snippet: Enriched CB CD34 + cells (2×10 4 /mL) and K562 cells (1×10 5 /mL) were transfected using the Human CD34 + Cell Nucleofection Kit and K562 Nucleofection Kit (Amaxa Biosystems, Koeln, Germany), respectively.

    Techniques: Expressing, shRNA, Transfection, Plasmid Preparation

    MKRN2 expression was down-regulated in CD34 + cells by nucleofection of shRNA. CD34 + cells expressing pGFP-V-RS-MKRN2 had a lower level of CFU-GEMM, compared with cells transfected with non-effective sh-pGFP-V-RS-NE or empty vector (pGFP-V-RS) (** P <0.01; n = 3).

    Journal: PLoS ONE

    Article Title: Ubiquitous Expression of MAKORIN-2 in Normal and Malignant Hematopoietic Cells and Its Growth Promoting Activity

    doi: 10.1371/journal.pone.0092706

    Figure Lengend Snippet: MKRN2 expression was down-regulated in CD34 + cells by nucleofection of shRNA. CD34 + cells expressing pGFP-V-RS-MKRN2 had a lower level of CFU-GEMM, compared with cells transfected with non-effective sh-pGFP-V-RS-NE or empty vector (pGFP-V-RS) (** P <0.01; n = 3).

    Article Snippet: Enriched CB CD34 + cells (2×10 4 /mL) and K562 cells (1×10 5 /mL) were transfected using the Human CD34 + Cell Nucleofection Kit and K562 Nucleofection Kit (Amaxa Biosystems, Koeln, Germany), respectively.

    Techniques: Expressing, shRNA, Transfection, Plasmid Preparation

    K562 cells were transduced with MKRN2 cDNA subcloned into lentiviral vector (pLEF1α-IG-MKRN2). MTT assay of pLEF1α-IG-MKRN2-transduced cells showed a significantly increased proliferation in culture, compared with pLEF1α-IG (empty vector) control cells (* P = 0.05; n = 3).

    Journal: PLoS ONE

    Article Title: Ubiquitous Expression of MAKORIN-2 in Normal and Malignant Hematopoietic Cells and Its Growth Promoting Activity

    doi: 10.1371/journal.pone.0092706

    Figure Lengend Snippet: K562 cells were transduced with MKRN2 cDNA subcloned into lentiviral vector (pLEF1α-IG-MKRN2). MTT assay of pLEF1α-IG-MKRN2-transduced cells showed a significantly increased proliferation in culture, compared with pLEF1α-IG (empty vector) control cells (* P = 0.05; n = 3).

    Article Snippet: Enriched CB CD34 + cells (2×10 4 /mL) and K562 cells (1×10 5 /mL) were transfected using the Human CD34 + Cell Nucleofection Kit and K562 Nucleofection Kit (Amaxa Biosystems, Koeln, Germany), respectively.

    Techniques: Transduction, Plasmid Preparation, MTT Assay, Control